中文摘要：
GABA能中間神經元被認為通過在不同的錐體細胞群上建立非選擇性連接來調節興奮性網絡，但最近的研究表明存在細胞類型特異性抑制性連接組。中間神經元如何以及何時在混合的興奮性神經元群之間建立精確的連接模式仍然是一個謎。我們探索了小鼠大腦皮層中細胞類型特異性抑制出現的分子機制。我們證明，第 5 層內 （L5 IT） 和端腦外 （L5 ET） 神經元表達特定的轉錄程序，使它們能夠塑造小白蛋白 （PV） 和膽囊收縮素陽性 （CCK） 中間神經元線路。我們確定了 Cdh12 和 Cdh13 這兩個鈣粘蛋白超家族成員，作為 L5 錐體細胞群細胞類型和輸入特異性抑制模式的基礎。多重單突觸追蹤揭示了 IT 和 ET 突觸前抑制網絡之間的重疊極小，并表明不同的 PV 籃子細胞群支配不同的 L5 錐體細胞類型。在這里，我們揭示了鈣粘蛋白在塑造細胞類型特異性皮質中間神經元布線中的貢獻。
英文摘要：
GABAergic interneurons were thought to regulate excitatory networks by establishing unselective connections onto diverse pyramidal cell populations, but recent studies demonstrate the existence of a cell type-specific inhibitory connectome. How and when interneurons establish precise connectivity patterns among intermingled populations of excitatory neurons remains enigmatic. We explore the molecular mechanisms orchestrating the emergence of cell type-specific inhibition in the mouse cerebral cortex. We demonstrate that layer 5 intra- (L5 IT) and extra-telencephalic (L5 ET) neurons express unique transcriptional programs, allowing them to shape parvalbumin- (PV+) and cholecystokinin-positive (CCK+) interneuron wiring. We identified Cdh12 and Cdh13, two cadherin superfamily members, as underpinnings of cell type- and input-specific inhibitory patterns of L5 pyramidal cell populations. Multiplex monosynaptic tracing revealed a minimal overlap between IT and ET presynaptic inhibitory networks and suggests that different PV+ basket cell populations innervate distinct L5 pyramidal cell types. Here, we unravel the contribution of cadherins in shaping cell-type-specific cortical interneuron wiring.
論文信息：
論文題目：Cadherins orchestrate specific patterns of perisomatic inhibition onto distinct pyramidal cell populations
期刊名稱：Nature Communications
時間期卷：16, Article number: 4481 (2025)
在線時間：2025年5月14日
DOI：doi.org/10.1038/s41467-025-59635-z
產品信息：
貨號：R-180, G-180
規格：100ul
品牌：Lumafluor
產地：美國
名稱：Fluorescent Retrobeads IX
辦事處：Target Technology(靶點科技)
Lumafluor對于可靠，穩健的逆行運輸，只有一個選擇：來自Lumafluor的RetroBeads™。來自Lumafluor的RetroBeads™-用于逆行追蹤的微球，以及在實驗中證明有效的無替代微球：綠色和紅色熒光RetroBeads™僅由Lumafluor提供。高度限制的注射-非常適合詳細的連接性研究。活細胞無限期（1年！），無毒。與大多數其他順行示蹤劑，原位雜交和免疫組織化學兼容。Retrobeads™IX：Retrobeads™向局部區域提供生物活性劑（如神經營養因子和神經遞質激動劑/拮抗劑）;逆行運輸允許確定哪些神經元暴露于藥劑[Riddle等。Nature378：189,1995和Quattrochi等。Science245：984,1989]。Retrobeads™IX專門用于促進蛋白質和其他生物活性化合物的吸附。與標準的Retrobeads™相比，Retrobeads™IX在靈長類動物系統中也是更有效的示蹤劑。美國Lumafluor逆向示蹤熒光微球Fluorescent Retrobeads IX見刊于Nature Communications：鈣粘蛋白將特定的圍體抑制模式編排到不同的錐體細胞群上
Lumafluor逆向示蹤熒光微球Fluorescent Retrobeads IX的材料和方法：
L5 pyramidal cell populations were targeted using green (488?nm) or red (555?nm) fluorescent retrobeads IX (Lumafluor Corp., FL). P2-3 pups were anesthetized with isoflurane (2.5%) and mounted on a stereotactic frame using a 3D printed isoflurane mask. Unilateral injections of 75?nl retrobeads at 30?nl/min were carried out as follows: L5 IT were labeled by targeting the contralateral somatosensory cortex (S1) (AP?+?1.6, ML ?1.9 to ?2.2, DV ?0.8 to ?0.5), L5 ET were labeled by targeting the Pons (AP ?0.3, ML?+?0.3, DV ?4.5 to ?4.0). Retrobeads were sonicated prior to each injection to avoid aggregate formation. The pipette was retracted from the brain after 2?min to allow for diffusion.
材料和方法文獻截圖：
